Figure 7: Expression of LSD1 in transgenic mice induces oxidative capacities in WAT.
From: LSD1 promotes oxidative metabolism of white adipose tissue
(a) Haematoxylin and eosin staining (H&E) of interscapular BAT, epididymal (epWAT) and inguinal (ingWAT) WAT of control (Ctrl) and LSD1 transgenic (Tg) mice. Black arrows indicate beige fat islets. Scale bar, 100 μm. (b) Ultrastructure analysis of ingWAT of Ctrl and Tg mice. Black arrows indicate mitochondria. LV, lipid vesicle. Magnified regions are delimited by squares. Scale bars, 5 μm (upper panels) or 2 μm (lower panels). (c) Mitochondrial density determined from ultrastructure analysis of ingWAT of Ctrl and Tg mice. (d) Determination of Sdh (mitochondrial respiratory chain complex II) and Cox (mitochondrial respiratory chain complex IV) activities in ingWAT of Ctrl and Tg mice. BAT of Ctrl mice was used as a positive control. (e) Relative transcript levels of the indicated markers in ingWAT of Ctrl and Tg mice. (a,d,e): n=10, (b,c): n=5; s.d. represents+s.e.m. Experiments (d,e) were independently repeated at least three times in triplicate. Statistical analysis was performed using two-tailed Student’s t-test. *P<0.05.
