Figure 3: Inertio-elastic focusing of bioparticles based on deformability and shape.
From: Inertio-elastic focusing of bioparticles in microchannels at high throughput
(a) Deformation statistics of WBCs in PBS, a low-molecular weight (357 kDa) HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR=ax/az. Scale bar, 10 μm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. (b) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q=13 ml min−1. (c) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q=20 ml min−1. Dashed red lines indicate channel centerline. Scale bar, 30 μm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
