Figure 2: Binding of MtbHU to different DNA substrates.
From: Targeting Mycobacterium tuberculosis nucleoid-associated protein HU with structure-based inhibitors
(a–f) Comparative EMSA showing the binding of increasing amounts of MtbHU to a fixed concentration of different DNA substrates. EMSA with (a) double-stranded linear DNA, (b) mismatch DNA, (c) gap DNA, (d) single-stranded DNA, (e) 3' overhang DNA and with (f) 5' overhang DNA. The DNA–protein complexes were resolved in 7% (w/v) non-denaturing polyacrylamide gels as described in Methods.
