Table 2 Binding affinities for DNA, SD1 and SD4 binding to MtbHU.

From: Targeting Mycobacterium tuberculosis nucleoid-associated protein HU with structure-based inhibitors

MtbHU

Kd for DNA (M)*†

K d for SD1 (M) ‡

K d for SD4 (M) ‡

Wild type

(6.4±0.4) × 10−9

(1.3±0.2) × 10−6

(9.8±1.3) × 10−8

Arg 55 Ala

(7.5±0.2) × 10−6

(1.1±0.4) × 10−5

(7.6±3.0) × 10−7

Arg 58 Ala

(8.8±0.2) × 10−6

(0.9±0.3) × 10−5

(9.8±5.2) × 10−7

Arg 80 Ala

(1.4±0.3) × 10−8

(0.7±0.8) × 10−5

(3.4±0.8) × 10−7

Lys 86 Ala

(8.5±0.13) × 10−9

(1.0±0.3) × 10−5

(7.3±2.3) × 10−7

  1. *Determined by SPR.
  2. †While the DNA-binding affinities were assayed by SPR, due to the hydrophobic nature of the inhibitor molecules, the inhibitor binding affinities were studied through fluorescence spectroscopy.
  3. ‡Determined by fluorescence spectroscopy.
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