Figure 7: Sequestration of GM3-containing AVN2 particles in mature DCs. | Nature Communications

Figure 7: Sequestration of GM3-containing AVN2 particles in mature DCs.

From: Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells

Figure 7

(a) The colour darkfield image of a representative DC after 1 h of incubation shows a distinct AVN cluster in a peripheral position. The overlay of (b) monochromatic darkfield and (c) LysoTracker images in (d) shows that AVNs are not sequestered into lysosomes. (e) Colour darkfield, (f) monochromatic darkfield and (g) fluorescence images of DCs co-incubated with Gag–eGFP VLPs for 1 h. (h) The overlay of darkfield and fluorescence images confirms that areas enriched in AVNs and VLPs colocalize. (i) Colour darkfield, (j) monochromatic darkfield and (k) fluorescence images of DCs incubated with AVNs and then fluorescently labelled for CD81. (l) The overlay images reveal that AVN-containing compartments are associated with CD81. Scale bars, 5 μm. All DC experiments were independently repeated with primary cells from at least two donors.

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