Figure 3: SRDX fusion restores the functions of EAR motif-deleted BZR1.
From: TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1
(a) Box diagram of BZR1, BZR1ΔEAR and BZR1ΔEAR-SRDX. (b,c) SRDX fusion restores both BZR1 promotion of hypocotyl elongation and inhibition of primary root growth. Seedlings were grown in the dark for 5 days on the medium containing 2 μM PPZ (b) or grown under light for 5 days on the medium containing either mock or 1,000 nM BL (c). The error bars indicate the s.d. (n=10 plants). Scale bar, 5 mm. (d,e) qRT–PCR analyses of BZR1-regulated genes. RNA was extracted from seedlings grown in the dark for 5 days on the medium containing 2 μM PPZ. Gene expression levels are normalized to that of PP2A and are shown relative to the expression levels in wild type. The error bars indicate the s.d. (n=3).
