Figure 3: Sox3-containing BAC clones caused XX sex reversal in transgenic fish.
From: Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena
(a) Transgenic BAC constructs covering the sex-determining locus. Numbers indicate ratios of transgenic lines producing sex-reversed XX males to total lines. The large BAC, BAC240, was made by joining two overlapping fosmid clones to IMBY09F23, and the reporter construct BAC240GFP was made by replacing the coding region of Sox3 gene with GFP (green) followed by a BGH polyadenylation signal (pA). (b–d) Representative images of the external secondary sexual characteristics in control XY, XX and transgene (BAC240)-positive XX fish. The secondary sexual characteristics are manifested in the size and shape of dorsal and anal fins (arrows). Both dorsal and anal fins of males are larger than those of females. The transgene-positive XX fish developed as sex-reversed males (158/259 individuals in total). Scale bars, 1 mm. (e–g) Representative images of gonadal histology in control XY males (n=2), XX females (n=2) and the transgene-positive XX males (n=2). The sex-reversed XX males had normal testes. Scale bars, 10 μm (e,g); and 100 μm (f).
