Figure 6: Amino-acid sequence alignment of DIPP-synthases. | Nature Communications

Figure 6: Amino-acid sequence alignment of DIPP-synthases.

From: X-ray structure of a CDP-alcohol phosphatidyltransferase membrane enzyme and insights into its catalytic mechanism

Figure 6

Alignment was done with Geneious 6.0.3 ref. 52 based on DIPPS sequences from 33 organisms with confirmed or putative DIPP activity12. The selected sequences represent the major branches of phylogenetic tree12. Examples of products from ipct/dipps genes fused (5) and separated (2). A., Archaeoglobus; T., Thermococcus; A., Aquifex; I., Ignisphaera; R., Rubrobacter; T., Thermotoga; and P., Pyrolobus. Strictly conserved residues among all IPCT/DIPPS sequences are displayed in bold, and from the consensus pattern of CDP-alcohol phosphatidyltransferase family are enclosed in a box. Similar and identical residues are marked, respectively, with a dot and an asterisk beneath, and mutated residues by a red M. Residues involved in pocket formation are highlighted with pink (1), orange (2) and blue (3). Secondary structure elements represented with red tubes (α-helices) were assigned with DSSP53.

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