Figure 7: Activity of the wild type IPCT/DIPPS and mutants assessed by 31P-NMR. | Nature Communications

Figure 7: Activity of the wild type IPCT/DIPPS and mutants assessed by 31P-NMR.

From: X-ray structure of a CDP-alcohol phosphatidyltransferase membrane enzyme and insights into its catalytic mechanism

Figure 7

The nuclear magnetic resonance spectra show the production of CDP-inositol and DIPP resulting from 30 min incubation at 85 °C of reaction mixtures containing 2.7 mM of CTP, 7 mM of inositol-1-phosphate and cell extract of E. coli producing A. fulgidus IPCT/DIPPS (0.9 mg of total protein). (a) Point mutation S308M; (b) point mutation S308A; and (c) wild type. Spectra were acquired in a Bruker Avance II 500 spectrometer.

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