Figure 2: Premature ageing of nfkb1^−/− mice.

(a) Kaplan–Meier survival curves (right censored) of wt and nfkb1^−/− mice, log-rank test, n⩾27. (b) Representative images of wt mice and an nfkb1^−/− mouse at the indicated ages showing premature hair greying and scruffy fur in nfkb1^−/− mice. (c) Age at maximum body weight in nfkb1^−/− and wt mice (n=56 for wt, n=8 for nfkb1^−/−, P<0.05, t-test). (d) Representative skin sections of 36-week-old animals, H&E stain. Boxed areas are shown at higher magnification. Mean epidermal thickness at the indicated ages is shown on the right (n⩾5). (e) Neuromuscular coordination measured as number of unsuccessful attempts to remain on a straight rod for 60 s (n=6 to 10 per group). All data are M±s.e.m. Significant differences (ANOVA with post hoc Holm–Sidak test, P<0.05) to respective controls of the same genotype are indicated by *, and between wt and nfkb1^−/− strains at the same treatment by #.