Figure 2: Locations of altered charged residues and measurements of DNA packaging dynamics. | Nature Communications

Figure 2: Locations of altered charged residues and measurements of DNA packaging dynamics.

From: Evidence for an electrostatic mechanism of force generation by the bacteriophage T4 DNA packaging motor

Figure 2

(a) Open book representation of the N- and C-terminal domain interface of gp17 (PDB ID code 3CPE) displaying the charged residues studied in this work. Changes in the residues labelled in grey resulted in zero bulk packaging while those labelled in black resulted in some packaging, leading to their further study through measurements with optical tweezers. Red indicates acidic interface residues, while blue indicates basic interface residues. (b–d) Representative measurements of single DNA molecule packaging dynamics (length packaged versus time) with (b) 5 pN applied load, (c) 30 pN and (d) 50 pN. The colours indicate WT (black), E537K (red), E537K-K305D (green) and E303K (blue) gp17 motors.

Back to article page