Figure 6: Mechanism of RBP-mediated regulation of MICB expression.
From: RNA-binding proteins regulate the expression of the immune activating ligand MICB
Relative mRNA abundance of MICB in RKO cells transduced with various shRNAs (a,c,e) or RBPs (b,d,f) was evaluated by qRT–PCR. hGAPDH was used as a reference gene. Shown are mean±s.e.m. of triplicates. Data are representative of three independent experiments. (a,c,e) The abundance of MICB in cells transduced with a control-shRNA was set as 1. (b,d,f) The abundance of MICB in cells transduced with STAU2 was set as 1. *P<0.05, by paired Students t-test. (g–i) Relative mRNA abundance of MICB in the shRNA-transduced RKO cells treated with ActD was evaluated by qRT–PCR. The abundance of MICB in each RBP–KD cell at time 0 was set as 1. The hGAPDH was used as a reference gene. Shown are mean±s.e.m. of triplicates. Data are representative of three independent experiments. *P<0.02, **P<0.002 by Students t-test.
