Figure 1: HOP2-MND1 does not prevent the accumulation of RAD51-ADP complexes, but stabilizes the filament in an active form. | Nature Communications

Figure 1: HOP2-MND1 does not prevent the accumulation of RAD51-ADP complexes, but stabilizes the filament in an active form.

From: HOP2-MND1 modulates RAD51 binding to nucleotides and DNA

Figure 1

(a) hRAD51 (10 μM) was added to ssDNA (#186, 30 μM) or to a mixture of ssDNA with mHOP2-MND1 (1 μM) in the presence of [α-32P]ATP and indicated metal ions. hRAD51 nucleoprotein complexes were isolated on Nytran filters and the ATP/ADP content was analysed by TLC39. (b) Graphical representation of data from (a). (c) Effect of Mg2+ concentrations on hRAD51-ADP accumulation was measured as in (a). The concentrations of Mg2+ and Ca2+ were 1 mM, each, or 50 μM (when indicated). (d) The effect of mHOP2-MND1 on the fluorescence of hRAD51 complexes with etheno M13 ssDNA in the absence (denoted as ‘no H2M1’) or presence of mHOP2-MND1 (0.2 μM) (denoted as ‘H2M1’). The experiments were repeated at least three times; error bars indicate s.e.m.

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