Figure 2: HOP2-MND1 strongly stimulates D-loop formation at low Mg2+concentration.
From: HOP2-MND1 modulates RAD51 binding to nucleotides and DNA
(a) The scheme of D-loop formation. The asterisk indicates 32P label. (b) Effect of mHOP2-MND1 (0.1 μM) on D-loop formation by hRAD51 (1 μM) with pUC19 scDNA (50 μM) and ssDNA (#186, 3 μM) in buffer containing Mg2+ at the indicated concentrations. D-loop formation was analysed by electrophoresis in a 1% agarose gel. Molecular size markers: D-loops (pUC19; 2,686 bp) and ssDNA (#186; 120 nt) are indicated by arrows. (c) Data from (b) are shown as a graph. The experiments were repeated at least three times; error bars indicate s.e.m.
