Figure 5: HOP2-MND1 stimulates the DNA strand exchange activity of RAD51 in the presence of ADP-BeF3, a ground-state ATP analogue. | Nature Communications

Figure 5: HOP2-MND1 stimulates the DNA strand exchange activity of RAD51 in the presence of ADP-BeF3, a ground-state ATP analogue.

From: HOP2-MND1 modulates RAD51 binding to nucleotides and DNA

Figure 5

(a) hRAD51 (1 μM) was incubated with ssDNA (#90, 3 μM) with or without mHOP2-MND1 (0.2 μM) in a reaction mixture containing either ADP-BeF3 or ADP-AlF4. D-loop formation was initiated by addition of pUC19 scDNA. Molecular size markers: D-loops (pUC19; 2686, bp) and ssDNA (#90; 90 nt) are indicated by arrows. (b) Binding of [α-14C]ADP-AlF4 and [α-14C]ADP-BeF3 by hRAD51 (10 μM) in the absence or presence of mHOP2-MND1 (1 μM) was analysed by filter binding. mHOP2-MND1, hRAD51 or mHOP2-MND1 and then hRAD51 were incubated with ssDNA (#71, 30 μM) in the presence of Mg2+ (0.1 mM) followed by filtration through Nytran membrane. The experiments were repeated at least three times.

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