Figure 2: Inhibition of IRE1α function by MKC9989 in human RPMI 8226 plasmacytoma cells. | Nature Communications

Figure 2: Inhibition of IRE1α function by MKC9989 in human RPMI 8226 plasmacytoma cells.

From: Structure and mechanism of action of the hydroxy–aryl–aldehyde class of IRE1 endoribonuclease inhibitors

Figure 2

(a) Time course analysis of MKC9989 inhibitory function on XBP1 splicing: reverse transcription PCR PAGE analysis (top) and quantification (middle) of XBP1-spliced products from three independent experiments shown as±s.e.m. Quantification of CD59 transcript levels from the same RNA samples (bottom). (b) Dose-response analysis of MKC9989 treatment on XBP1 splicing in human RPMI 8226 plasmacytoma cells. Shown is a representative profile with EC50 calculated as the average of two independent experiments.

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