Figure 2: SIM2 and SIM3 are the key determinants for SUMO-2 chain binding and ubiquitination. | Nature Communications

Figure 2: SIM2 and SIM3 are the key determinants for SUMO-2 chain binding and ubiquitination.

From: Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4

Figure 2

(a) Pull-down experiment with MBP-tagged SIM mutants of RNF4. Bound material was analysed by SDS–PAGE and Coomassie blue staining (lower panel) or by immunostaining with anti-SUMO-2 antibody (upper panel). (b) Substrate ubiquitination activity of SIM mutants of RNF4. Data represent the mean of duplicate reactions with errors displayed as±s.d. Coomassie blue-stained SDS–PAGE gel with wild-type and mutant RNF4 proteins is shown as a loading control. Mutated SIMs are marked by X.

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