Figure 1: NANOG expression in mouse adult tissues.
From: Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia
(a) H&E staining, IHC for NANOG and ISH using a Nanog RNA probe of the indicated adult tissues from C57BL/6 mice. Two magnifications are shown for each tissue. (b) IHC for NANOG, SOX2, OCT4 and P63 of the indicated adult tissues from C57BL/6 mice. Stomach corresponds to forestomach. Tongue shows the dorsal (upper) and ventral (lower) parts. (c) Immunoblot of NANOG extracts from the indicated tissues from C57BL/6 mice. Extracts from wild-type (+/+) and Nanog knockout (−/−) ESCs are included as positive and negative controls and the amount loaded is one-tenth the amount loaded for the tissues. GAPDH was used as loading control. Eso, oesophagus; Fs, forestomach; Liv, liver; Mu, muscle, MW, molecular weight. (d) Methylation status of the Nanog promoter determined by pyrosequencing. Microdissected epithelial (Epi) and muscular (Mu) layers from forestomach (Fs) and oesophagus (Eso) from C57BL/6 mice (n=3 mice) were analysed. Liver was also analysed as a NANOG-negative tissue. MEFs (two independent isolates) and ESCs (two independent lines) were used as positive and negative controls, respectively. Values correspond to mean±s.e.m. Statistical significance was determined by the two-tailed Student’s t-test comparing epithelial versus muscular layers: *P<0.05 and ***P<0.001. In a and b, scale bars, 50 μm.
