Figure 3: NANOG overexpression causes hyperplasia in stratified epithelia.

(a) H&E staining in the oesophagus (left) and forestomach (right) upon NANOG overexpression showing epithelial hyperplasia. CTR and TG mice were continuously treated with low or high DOX. Histological analyses were performed at the humane endpoint of TG mice (~2 months) or at the end of the observation period for CTR mice (11 months). (b) H&E and IHC of the indicated proteins in oesophageal serial sections of CTR and TG mice treated for 2 weeks with high DOX. (c) CTR and TG mice treated for 2 weeks with high DOX were injected intraperitoneally with BrdU 2 h prior to killing and oesophageal serial sections were analysed by IHC against NANOG and BrdU. In (a–c), pictures in the lower rows correspond to magnifications of selected areas in the upper pictures (marked with a dotted rectangle). Scale bars, 100 μm.