Figure 6: Nanog and Aurka induce proliferation and aneuploidy.

(a) IHC of AURKA and the indicated proliferation markers in control Col1a1^+/+; Rosa26^rtTA/+ mice (A-CTR) and Aurka-inducible Col1a1^tetO-Aurka/+;Rosa26^rtTA/+ (A-TG) littermate mice treated with high DOX for 48 h. The graphs below correspond to the percentage of AURKA, Ki67, BrdU and pH3-positive nuclear areas for each condition in the basal (bas) and suprabasal (sb) epithelial layers. (b) H&E staining, and IHC for AURKA and BrdU in oesophageal serial sections of A-CTR and A-TG mice treated for 2 weeks with high DOX and injected intraperitoneally with BrdU 2 h before killing. The graph shows the percentage of AURKA- and BrdU-positive nuclear areas for each condition in the basal (bas) and suprabasal (sb) epithelial layers. (c) Representative images of the oesophagus basal cells of CTR, Nanog-overexpressing (TG) and AurkA-overexpressing (A-TG) mice treated 2 weeks with high DOX, and hybridized against a chromosome 11 specific probe (white arrows). Dotted white lines mark the epithelial basal layer. Graph shows the quantification of cells showing more than two signals for the chromosome 11 probe. A total of 49 to 64 cells was quantified per group from a total of three CTR and TG mice, or one A-TG mouse. Statistical significance for the presence of more than two FISH signals (>2 signals) was determined using a two-sided χ² test: *P<0.05. In a and b, scale bars correspond to 50 μm; in c, scale bars correspond to 10 μm. In a and b, values correspond to mean±s.e.m. of the indicated number of mice (n), and statistical significance relative to CTR was determined by the two-tailed Student’s t-test: *P<0.05; **P<0.01; ***P<0.001.