Figure 7: NANOG is overexpressed in mouse and human squamous cancers.
From: Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia
(a) NANOG IHC in archived mouse tumours. mPIN, mouse prostate intraepithelial neoplasia; (b) IHC of NANOG and AURKA in two representative human ESCCs. (c) IHC of NANOG and AURKA in two representative HNSCCs. (d) Immunoblots of HNSCC cells (SCC38 and SCC42B) infected with lentiviruses carrying scramble (shSCR) or anti-NANOG (shN) shRNAs. Samples were analysed 5 days after infection and selection. NTERA2 (NT2) cells were used as a positive control for NANOG. γ-tubulin (γ-TUB) was used as a loading control. MW, molecular weight. (e) Cells as in d were subjected to 1 h EdU pulse, Hoechst stained and analysed by flow cytometry. Their proliferation rate was quantified as the percentage of EdU-positive cells, see graph in the upper part of the panel. Values correspond to mean±s.e.m. of three (for SCC38) or two (for SCC42B) independent biological replicates. Statistical significance was determined by the two-tailed Student’s t-test: **P<0.05; ***P<0.001. A representative flow cytometry plot is shown in the lower part of the panel. In (a–c), two magnifications are shown for each tissue and scale bars correspond to 50 μm.
