Table 2 CRISPR/Cas-mediated genome editing in F1 hybrids.

From: Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR–Cas platform

Microinjection

Embryos injected*

Two-cell embryos (%)

Pups delivered (%)

Tyrc-KO (%)

TyrC-KO (%)

Cas9+gRNA:Tyrc

104

48 (46.2)

21 (43.8)

6 (28.6)

0 (0)

Cas9+gRNA:TyrC

135

71 (52.6)

23 (32.4)

0 (0)

7 (30.4)

  1. *F1 embryos collected from the superovulated F344 female previously mated with DA males.
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