Table 3 CRISPR/Cas-mediated genome editing with ssODNs in F344 rats.
Microinjection | Embryos injected | Two-cell embryos (%) | Pups delivered (%) | Tyr | Asip | Kit | ||||
|---|---|---|---|---|---|---|---|---|---|---|
KO (%) | KI (%) | KO (%) | KI (%) | Kit-1 KO (%) | Kit-2 KO (%) | KI (%) | ||||
Cas9+gRNA:Tyrc+ssODN:TyrC | 94 | 45 (47.9) | 13 (28.9) | 3 (23.1) | 1 (7.7) | – | – | – | – | – |
Cas9+gRNA:Asipa+ssODN:AsipA | 195 | 91 (46.7) | 33 (36.3) | – | – | 5 (15.2) | 6 (18.2) | – | – | – |
Cas9+gRNA:Kith-1+gRNA:Kith-2+ssODN:KitH | 104 | 43 (41.3) | 25 (58.1) | – | – | – | – | 9 (36.0) | 0 (0) | 1 (4.0) |
