Figure 5: IL-23 upregulates IL-23R to further promote IL-23-induced apoptosis. | Nature Communications

Figure 5: IL-23 upregulates IL-23R to further promote IL-23-induced apoptosis.

From: IL-23 promotes TCR-mediated negative selection of thymocytes through the upregulation of IL-23 receptor and RORγt

Figure 5

(a) Flow cytometry analysis of IL-23R on the indicated thymocyte populations from WT B6 mice at the indicated times after Ad virus administration. The percent of IL-23R+ cells is shown. (b) Immunofluorescent assessment of IL-23R expression by anti-IL-23R (green) and apoptosis (TUNEL, red) of thymic sections from the indicated mice. Sections were co-stained with A647 anti-CD90.2 (blue) and A555 anti-EpCAM (white). Magnification is shown below the images. (c) Immunofluorescent assessment of IL-23R-GFP expression (green) on thymus sections obtained from heterozygous Il23r+/−-GFP KI mice 5 days after either AdLacZ or AdIL-23 administration. Sections were co-stained with anti-CD90.2 (red) and anti-EpCAM (white). Magnification is indicated to the right of the images. (d) A single green channel higher power view of the outlined area from c. N=4–5 mice per group for three independent experiments. Scale bar, 50 μm, applies to all panels.

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