Figure 6: CD4hiCD8hi DP thymocytes are targets of IL-23R-RORγt-mediated apoptosis. | Nature Communications

Figure 6: CD4hiCD8hi DP thymocytes are targets of IL-23R-RORγt-mediated apoptosis.

From: IL-23 promotes TCR-mediated negative selection of thymocytes through the upregulation of IL-23 receptor and RORγt

Figure 6

AdLacZ or AdIL-23 was administered to WT (Rorc+/+) or Rorc+/− mice. (a) Quantitative real-time PCR (qRT–PCR) analysis of Rorc and Il23r on the indicated thymocytes of Rorc+/+ mice at day 5. (b) Left: Gross anatomic images showing the size of a representative thymus of WT (Rorc+/+) or Rorc+/− mice at day 9. Right: Flow cytometry analysis of CD4 and CD8 on total thymocytes (Thy1.2+). The frequency of CD4+CD8+ cells within the total Thy1.2+ thymocytes is indicated. Scale bar, 3.0 mm. (c) qRT–PCR analysis of Il23r on the indicated thymocyte subpopulations from the indicated mouse strains at day 5. (d) qRT–PCR analysis of the expression of c-Rel and Bcl-xl in the indicated thymocyte subpopulations at day 5. Data in (a–d) are representative of three independent experiments (mean±s.e.m., N=3–5 mice per group; **P<0.01, ***P<0.005 between AdLacZ and AdIL-23-treated or Rorc+/− and Rorc+/+ mice).

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