Figure 5: Wps mutation causes mitochondrial dysfunction in adipose tissues.
From: The stem cell factor/Kit signalling pathway regulates mitochondrial function and energy expenditure
(a) Representative TEM images of BAT from 5-month-old mice. Scale bar, 1 μm. (b) Size, number and total area of BAT mitochondria (n=3–4). (c) Relative mtDNA copy number in BAT from weight-matched 10–12-week-old mice (n=4). (d) Mitochondrial respiration rate in BAT from weight-matched 10–12-week-old mice (n=4). (e) Real-time quantification of Ppargc1a mRNA levels in BAT (n=4). (f) Immunobloting of PGC-1α protein of BAT lysate from 6-week-old mice. (g) Relative Tfam mRNA levels in BAT (n=4). (h) Relative mRNA and (i) protein levels of PGC-1α in WAT (n=4–5). (j) Relative expression of Tfam mRNA level in WAT (n=4–5). (k) mtDNA copy number in WAT from weight-matched mice (n=7). Data are shown as mean±s.e.m. *P<0.05; **P<0.01 by two-tailed Student’s t-test.
