Figure 6: Wps mutation causes mitochondrial dysfunction in soleus muscle.
From: The stem cell factor/Kit signalling pathway regulates mitochondrial function and energy expenditure
(a) Representative TEM image of soleus muscle from 5-month-old mice. Scale bar, 2 μm. (b) Size, number and total area of mitochondria in soleus muscle (n=3–4). (c) mtDNA copy number in soleus muscle of weight-matched 10–12 week-old mice (n=4). (d) ADP-stimulated state 3 respiration of isolated soleus mitochondria from weight-matched 10–12 week-old mice (n=3). (e) Real-time quantification of Ucp1 mRNA levels in soleus muscle (n=4–5). (f) Immunoblotting of PGC-1α in soleus muscle of 6 week-old mice. (g) Relative Tfam mRNA levels in soleus muscle (n=4–5). Data are shown as mean±s.e.m. *P<0.05; **P<0.01 by two-tailed Student’s t-test.
