Figure 8: SCF overexpression increases thermogenesis.
From: The stem cell factor/Kit signalling pathway regulates mitochondrial function and energy expenditure
(a) Soluble and total Kitl mRNA levels in BAT and (b) serum SCF levels from overnight-fasted and 3 h refed mice (n=4). (c) Soluble and total Kitl mRNA levels in BAT and (d) serum SCF levels from mice exposed to ambient temperature (22 °C) or cold (6 °C) for 24 h (n=4). (e) SCF level in BAT from cold-challenged mice (n=4). (f) Soluble and total Kitl mRNA levels in BAT from mice acclimated at thermoneutrality (30 °C) or cold (4 °C) for 7 days. (g,h) Lentiviruses expressing EGFP (n=6) or SCF (n=11) were injected into interscapular BAT of 8-week-old male C56BL/6 mice (1 × 106 transforming units per site). Four weeks later, gene expression in BAT (g) and body temperature during cold exposure (h) were determined. (i–l) Lentiviruses expressing EGFP (n=4) or SCF (n=5) (1 × 106 transforming unit per mouse) were administrated via the tail vein. Serum SCF level (i), energy expenditure (j), body temperature during cold exposure (k) and body weight (l) were determined 5 weeks later. Data are shown as mean±s.e.m. *P<0.05; **P<0.01; ***P<0.001 by two-tailed Student’s t-test.
