Figure 2: Pr55Gag binding to genomic and spliced HIV-1 RNAs.
From: Specific recognition of the HIV-1 genomic RNA by the Gag precursor
(a) Schematic drawing of the first 600 nucleotides of genomic and 5 different spliced HIV-1 RNA fragments used. The relative binding affinity of Pr55Gag to these RNAs is indicated on the right. (b) Binding of Pr55Gag (left panel) and mature NCp7 (right panel) to these RNAs was evaluated by filter binding. Data are represented as mean±s.e.m (n=3 or 4). (c) Assessment of the gRNA binding specificity to Pr55Gag using gel mobility shift assay. Radiolabelled N1-600WT RNA was incubated together with 100 nM Pr55Gag and increasing amounts of unlabelled genomic or spliced vRNA as indicated above the gels. The first two lanes correspond to radiolabelled N1-600WT RNA incubated under denaturing and renaturing conditions, respectively. The concentration of unlabelled competitor RNA in lanes 3 to 9 was 0, 10, 50, 100, 200, 400 and 800 nM, respectively. The last panel presents a quantification of the gels. The fraction of bound RNA corresponds to the sum of all complexes.
