Figure 3: Pr55Gag binding to different regions of the HIV-1 gRNA.
From: Specific recognition of the HIV-1 genomic RNA by the Gag precursor
(a) Schematic drawing of the gRNA fragments. The binding affinity of Pr55Gag to these RNAs, normalized relative to RNA N1-600WT or M1-615WT, is indicated on the right. Binding of Pr55Gag to large RNAs derived from the gRNA of the NL4-3 (b) and MAL (c) isolates was evaluated by filter binding. Data are represented as mean±s.e.m. (n=3 to 8). (d) Binding of Pr55Gag to the individual SL motifs was analysed by band-shift assays. The structure of the various SL motifs is shown above the gels; two short RNAs corresponding to the apical part of SL1 (NapSL1) and the full-length SL1 (NflSL1) were tested. The Pr55Gag concentration in lanes 1 to 6 was 0, 50, 100, 200, 400 and 600 nM, respectively. M and D correspond to the monomeric and dimeric species, respectively, of NflSL1 and NapSL1.
