Figure 4: Spectral analysis of light distribution in the guinea pig retina.

(a) Twenty-seven images of transmitted light were recorded above the photoreceptors layer. Three representative images for short- (450 nm), middle- (510 nm) and long wavelengths (560 nm) are shown. The images were normalized (the sum of all pixels is equal for all images). Müller cells light tubes were located by a threshold and the 10 highest transmission cells were marked and masked. All these cells, without exception, show a higher transmission of red-green wavelengths (b, inset). (b) Mean (±s.d.) of the spectral light distribution inside Müller cells (N=10), studied in the guinea pig retina #1. Individual traces are overlaid in the inset. (c) Normalized light concentration spectra inside Müller cells (different colours), and in their surrounding areas (e) were obtained from five different retinal preparations. The mean (±s.d.) of the concentration spectra of light transmitted inside Müller cells, and in their surrounding in all studied retinas (N=5) correspond well with the concentration spectra obtained using the model simulation (purple curve) (d and f, respectively). All panels were normalized to unity at maximum.