Figure 5: Instant 3D zoom-in imaging and quantitative measurement of single-nucleus gene expression for C. elegans. | Nature Communications

Figure 5: Instant 3D zoom-in imaging and quantitative measurement of single-nucleus gene expression for C. elegans.

From: Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis

Figure 5

(a) A 3D pre-scan image of L1 C. elegans, along with the instant 3D measurements of gene expression levels of three cells BWMDL23, DEP and BWMDL24. p: location of interest for zoom-in imaging at a higher resolution. (b) Digital zoom-in of the area around p, where the insufficient voxel resolution does not manifest clear nuclei boundary. (c) Optical zoom-in around p, where the boundary between several nuclei is visible. (d) Instant 3D measurement of the gene expression of two bundles of body wall muscle cells, without computational segmentation of cells. For the profile of each curve of c1 and c2, the top is the gene expression of channel 1 (red) and bottom is that for channel 2 (green). Red: Punc54::H1::cherry. Green: Pmyo3::eGFP.

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