Figure 6: Instant 3D microsurgery for different animals. | Nature Communications

Figure 6: Instant 3D microsurgery for different animals.

From: Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis

Figure 6

(a) Instant 3D pinpointing of body wall muscle cells in a fixed L1 C. elegans worm. p1, p2, p3: three muscle-cell nuclei. Red: Punc-54::H1::mCherry. Green: Pmyo-3::eGFP. (b) Instant 3D bleaching of the muscle cell nuclei in a. (c) Instant 3D pinpointing of muscle cells in a live L1 C. elegans worm. p4, p5: two muscle cells. Red: Pmyo-3::tagRFP-T. Green: Pmyo-3::GCaMP3. (d) Instant 3D bleaching of the muscle cells in c leads to bending of the animal. (e) Instant 3D pinpointing for an ato-GAL4-labelled Drosophila brain. p6: a cell body of a neuron. p7, p8: two loci on a major ato-GAL4 neurite tract. Green: ato-GAL4 pattern. (f) Instant 3D bleaching of locations in e and instant 3D curving for the same specimen. c1: a 3D curve cutting through the arbor of ato-GAL4 pattern in the optic lobe. (g) Instant 3D bleaching of the c1 curve in f.

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