Figure 7: Instant 3D visualization of massive 3D image data stacks. | Nature Communications

Figure 7: Instant 3D visualization of massive 3D image data stacks.

From: Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis

Figure 7

(a) Visualization of a 2.52-Tb whole-mouse brain image stack, which has 30,000 × 40,000 × 700 voxels and three 8-bit fluorescent channels. For each scale (S0~S4) when a user is using 3D VF’s one mouse stroke feature to zoom-in at arbitrarily defined 3D ROI, the ROI-computing time and the actual 3D rendering time for this ROI are also shown on top of each magenta arrow. (b) Bench-test of ROI-computing time (mean±s.d.) for five different large image stacks of different properties on different operating systems (Mac, Linux and Windows). The five images are: single mouse neuron (22.8 Gb, 16 bit, single channel, 11.4 Gigavoxels), two rat neurons (96.0 Gb, 8 bit, two channels, 48.0 Gigavoxels), hippocampus (330.3 Gb, 8 bit, single channel, 330.3 Gigavoxels), whole-mouse brain (504 Gb, 8 bit, 3 channels, 168 Gigavoxels) and another whole-mouse brain (2.52 Tb, 8 bit, 3 channels, 840 Gigavoxels). See Methods for the configurations of machines used in these tests. (c) The actual 3D rendering time to visualize the image contents in each computed ROI, bench tested for the same data sets in b and various operating systems.

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