Figure 6: Intratracheal (i.t.) instillation of rhMG53 protects PPE-induced injury to rat lung.
From: Treatment of acute lung injury by targeting MG53-mediated cell membrane repair
(a) PPE was applied through i.t. spray. LDH in BALF was measured 30 min after PPE application to evaluate cell damage. Different concentrations of MBP-MG53 or control proteins (denatured MBP-MG53 or BSA) were applied through i.t. spray 5 min before PPE. 0.2 and 2 mg kg−1 MBP-MG53 significantly reduced LDH in BALF. The measurements of chronic responses were conducted 4 weeks after PPE application with repetitive application of MBP-MG53 (i.t., 5 days per week for 4 weeks). EELV (b), lung elastance (c) and alveolar structure (quantified by Lm) of the lung (d) were compared with control treatment groups. n=7–10 per group. (e) Representative histology images of lung parenchyma in PPE-induced injury to rat lung with or without administration of MG53 (scale bars, 200 μm). *P<0.05 versus saline control group (open bar). #P<0.05 versus denatured MBP-MG53 group (Mann–Whitney U-analysis).
