Figure 4: Genetic inhibition of Nox2 activity ameliorates pathological and functional phenotypes in dystrophic muscle. | Nature Communications

Figure 4: Genetic inhibition of Nox2 activity ameliorates pathological and functional phenotypes in dystrophic muscle.

From: Src-dependent impairment of autophagy by oxidative stress in a mouse model of Duchenne muscular dystrophy

Figure 4

(a) Haematoxylin and eosin staining of cross-sections from Dia showing central nuclei (arrow head) and smaller fibres (arrow). (b) Immunoblot analysis of macrophage content (CD68). (c) Immunofluorescent and bright field images of Dia cross-sections showing fibre-type distribution. Type I (red), IIA (green), IIB/IIX ( white x, unstained and viewed from bright field overlay). (d) Serum creatine kinase activity. (e) Force–frequency relationship in Dia muscle strips from WT (black), mdx (red) and p47−/−-mdx (blue) mice. Scale bars represent 55 μm. For e, #P<0.01 p47−/−-mdx versus mdx.##P<0.01 p47−/−-mdx versus WT and mdx. Mdx was statistically different than WT at all frequencies of stimulation. Statistical differences between groups were determined using ANOVA with Tukey’s post hoc test. *P<0.05 and **P<0.01.

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