Figure 3: Enzymatic and chemical release shows that MAR synthesis is the primary activity of non-H-Y-E PARPs. | Nature Communications

Figure 3: Enzymatic and chemical release shows that MAR synthesis is the primary activity of non-H-Y-E PARPs.

From: Family-wide analysis of poly(ADP-ribose) polymerase activity

Figure 3

Automodified PARPs were treated with CHES or the indicated wild type and catalytically inactive ADPr hydrolytic enzyme. Signal remaining attached to protein was analysed by SDS–PAGE (top row), Coomassies for each PARP are shown below. Released product was analysed by TLC (middle row) and sequencing gel (bottom row). Assays were repeated at least twice. CHES, MacroD1 and T. curvata PARG treatment of non-H-Y-E PARPS results in ADPr release, indicative of MAR synthesis activity. See also Supplementary Figs 4,5.

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