Figure 3: Filamin plays a crucial role in apical extrusion of v-Src-expressing cells in the EVL of zebrafish embryos.
From: Filamin acts as a key regulator in epithelial defence against transformed cells
(a) Confocal images of a GFP-filamin-expressing zebrafish embryo (at 10-11 h post fertilization) expressing v-Src in a mosaic manner. Embryos from an EVL-specific GFP-filamin A-expressing line were injected with the UAS:Cherry-v-Src-expressing vector at the one-cell stage. Arrows indicate GFP-filamin accumulation in the neighbouring EVL cells at the interface with v-Src-expressing EVL cells (red). White dashed lines in xy panels denote the cross-sections represented in xz panels. (b) Effect of filamin morpholino oligonucleotides (MO) on apical extrusion of v-Src-expressing cells. Embryos were injected with the v-Src-expressing vector and indicated oligonucleotides. Representative images were shown on the right. Arrowheads indicate v-Src-expressing extruded cells. Data are mean±s.e. from ten embryos for control MO and from nine embryos for filamin MO1. *P=1.0 × 10−5 (Student’s t-test). Scale bars, 10 μm (a), 20 μm (b). (c) Rescue of the effect of filamin MO on apical extrusion by expression of exogenous filamin A. Embryos were injected with the v-Src-expressing vector, indicated oligonucleotides and vector. Note that v-Src and GFP-filamin were specifically expressed in the EVL. Data are mean±s.e. from 12 embryos for control MO+GFP, from 10 embryos for filamin MO1+GFP and from 12 embryos for filamin MO1+GFP-filamin. *P=0.0023, **P=0.0024 (Student’s t-test).
