Figure 7: The Rho-Rho kinase pathway in the surrounding normal cells is involved in filamin accumulation at the boundary with transformed cells.

(a) Effect of Y27632 on filamin accumulation at the interface with RasV12-MDCK cells. Data are mean±s.e. from two independent experiments. *P=0.044 (Student’s t-test ). (b) Effect of Y27632 on filamin accumulation at the interface with ts-Src MDCK cells. Data are mean±s.e. from three independent experiments. Values are expressed as a ratio relative to dimethyl sulphoxide (DMSO). *P=0.0045 (Student’s t-test). (c,d) Effect of overexpression of RhoGAP in the surrounding cells on apical extrusion of RasV12-MDCK cells (c) or filamin accumulation at the interface with RasV12-MDCK cells (d). Data are mean±s.e. from two independent experiments. Values are expressed as a ratio relative to MDCK:Ras=50:1 (d). *P=0.011 (c), *P=2.8 × 10⁻⁴ (d) (Student’s t-test). (e,f) Effect of overexpression of RhoGAP in the surrounding cells on apical extrusion of ts-Src MDCK cells (e) or filamin accumulation at the interface with ts-Src MDCK cells (f). Data are mean±s.e. from three independent experiments. Values are expressed as a ratio relative to tet (−) (f). *P=0.041 (e), *P=2.0 × 10⁻⁴ (f) (Student’s t-test). (g,h) Effect of overexpression of a dominant negative mutant of Rho kinase in the surrounding cells on apical extrusion of RasV12-MDCK cells (g) or filamin accumulation at the interface with RasV12-MDCK cells (h). Data are mean±s.e. from three (g) or two (h) independent experiments. Values are expressed as a ratio relative to MDCK:Ras=50:1 (h). *P=0.0040 (g), *P=0.033 (h) (Student’s t-test). (i,j) Effect of overexpression of a dominant negative mutant of Rho kinase in the surrounding cells on apical extrusion of ts-Src MDCK cells (i) or filamin accumulation at the interface with ts-Src MDCK cells (j). Data are mean±s.e. from five (i) or three (j) independent experiments. Values are expressed as a ratio relative to MDCK:Src=50:1 (j). *P=0.040 (i), *P=0.011 (j) (Student’s t-test).