Figure 2: Intramolecular BRET signals of Rluc-PTEN-YFP.
From: A biosensor to monitor dynamic regulation and function of tumour suppressor PTEN in living cells
(a) mBRET measurements in HEK293 cells transfected with Rluc-PTEN-YFP for intramolecular BRET, or cotransfected with Rluc-PTEN and PTEN-YFP for intermolecular BRET. PC-3 cells were transfected with Rluc-PTEN-YFP at levels comparable to endogenous PTEN in HEK cells as shown in western blotting (b). Data are mean±s.e.m. of four independent experiments. (c) BRET measurements for increasing concentrations of the indicated constructs. Data are mean±s.e.m. of four independent experiments. (d) Representative blots using an anti-PTEN antibody. Symbols (*) and (+) refer to their respective BRET measurements in c. BRET measurements in these experiments were performed on detached cells.
