Figure 6: S1PR2-induced PTEN conformational rearrangement monitored by real-time BRET.
From: A biosensor to monitor dynamic regulation and function of tumour suppressor PTEN in living cells
HEK (a) or HeLa cells (b) were cotransfected with Rluc-PTEN-YFP and S1PR1 or S1PR2, and treated with S1P (100 nM) or vehicle (PBS). Graphs represent ΔmBRET measurements at t=5 min as mean±s.e.m. for four independent experiments. **P<0.01, t-test, n.s., not significant. (c) HeLa cells were preincubated with the S1PR2-specific antagonist JTE013 (1 μM) and then treated with S1P for 5 min. Cells were lysed and assessed for active AKT (pAKT) levels. BRET measurements in these experiments were performed on adherent cells.
