Figure 2: Inhibition of CaV1.342 current by compound 8 and nimodipine (Nimo).
From: Modest CaV1.342-selective inhibition by compound 8 is β-subunit dependent
(a) Representative traces of CaV1.342 currents recorded by single 1 s square pulse to test potential of 10 mV from holding potential of −70 mV for 25 sweeps with sweep interval of 20 s. Only the first three untreated (black) and last three treated (red) traces are shown for each treatment groups. Displayed are the first 100 ms of each recording trace. (b) Averaged diary plot of effects of either control, 5 μM 8, 50 μM 8 or 5 μM Nimo on CaV1.342 peak currents. The number of cells analysed are indicated in the parenthesis. The drugs were added after the three stable sweeps. (c) Population data of the % peak current inhibition at the 25th sweep normalized against the first sweep conferred by either 5 μM 8, 50 μM 8 or 5 μM Nimo as compared with the control. NS, non-significant. ***P<0.001 (Student’s unpaired t-test). Alternatively, P<0.001 among all the treatment groups (one-way analysis of variance and Bonferroni’s test). The number of cells analysed is indicated in b. The data for each condition were collected from two to three transfections.
