Figure 6: Inhibition of CaV1.342, CaV1.342_UC, CaV1.2B15 and CaV1.342a current by 50 μM compound 8 in the presence of different β-subunits.
From: Modest CaV1.342-selective inhibition by compound 8 is β-subunit dependent
(a) Averaged diary plot of effects of 50 μM 8 on CaV1.342 peak currents in the presence of β1-, β2a-, β3- and β4-subunit. The current was recorded by single square pulse from holding of −70 to 10 mV for over 100 ms with sweep interval of 20 s. (b–d) Averaged diary plot of effects of 50 μM 8 on the peak currents of CaV1.342_UC, CaV1.2B15 and CaV1.342a, respectively, in the presence of different β-subunits, format as in a. (e) Comparison of the % peak current inhibition at the 28th sweep normalized against the first sweep conferred by 50 μM 8 for CaV1.342, CaV1.342_UC, CaV1.2B15, CaV1.342a in the presence of different β-subunits. Within each β-subunit, the % inhibitions of CaV1.342_UC, CaV1.2B15, CaV1.342a were compared with that of CaV1.342. NS, non-significant, *P<0.05, **P<0.01, ***P<0.001 (Student’s unpaired t-test). Alternatively, P<0.001 within each group (one-way analysis of variance and Bonferroni’s test). The number of cells analysed is indicated in b–d. The data for each condition were collected from two to three transfections.
