Figure 3: miR-210 regulates gastric cell proliferation.

(a) The indicated gastric cells were transfected with 50 nM of miR-210 or miR-210 mut (mutant) and assessed for cell viability 4 days later. The data indicate average values calculated from triplicates. Error bars show s.d. (n=3). (b) The gastric cell lines were transfected with 50 nM of anti-miR-Nega or anti-miR-210 and assessed for cell viability 4 days later. The data shown are average values calculated from triplicates. Error bars show s.d. (n=3). (c) MKN45 cells were transfected with the indicated concentration of miR-210 or mutant miR-210 and assessed for cell viability 4 days later. The error bars indicate s.d. (n=3). (d) MKN45 cells were transfected with an miRNA-negative control, miR-210 or a mutant miR-210. After 2 days, the cells were stained with propidium iodide and analysed by flow cytometry. *P<0.05, **P<0.01, ***P<0.001, NS, not significant by Student’s t-test.