Figure 1: Schematic representation of the circular crAssphage genome.
From: A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes
The genome contains 80 ORFs that were predicted with Glimmer56 trained on Caudovirales. The total coverage of each nucleotide in the F2T1 metagenome, and in all public metagenomes in MG-RAST49 is indicated (466 human faecal and 2,440 other metagenomes, as determined by blastn mapping: ⩾75 bp aligned with ⩾95% identity, see Methods). Green bars indicate the 36 regions that were validated by long-range PCR (see Table 2 and Supplementary Table 1). Selected regions of several PCR amplicons (indicated as light green regions in the green bars) were sequenced by Sanger dideoxynucleotide sequencing to validate that the amplicons were indeed derived from the crAssphage genome (Supplementary Table 1). See Supplementary Fig. 6 for the fully annotated figure.
