Table 2 PCR primer pairs designed to validate the crAssphage genome sequence.
From: A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes
Nr | Forward primer sequence | Start | Reverse primer sequence | End |
|---|---|---|---|---|
1 | 5′-GTGACGAGAGGTATTGAATGTGGA-3′ | 1,785 | 5′-GCTATAAGTCCAGCAGCAAAAGG-3′ | 6,793 |
2 | 5′-TGACTAGCTTGCTTCCATCCT-3′ | 6,004 | 5′-GCACTACGTCCATCTTGAGTACCA | 11,923 |
3 | 5′-CAGGTGAACGTAAACCTGTTCCT-3′ | 9,512 | 5′-ACTCATACCAGCAAATGAAGGCA-3′ | 14,930 |
4 | 5′-ATGGTGCTCGTGAAATTGCT-3′ | 13,526 | 5′-GCTTTACGCTGAGCAATCGT | 17,858 |
5 | 5′-GCACCGGTATTGCAAAGGCT-3′ | 17,801 | 5′-CTCCAAATCCTTTGTTTCCACGT-3′ | 25,822 |
6 | 5′-TGCTATTTGGCAAACTGCTGG-3′ | 23,445 | 5′-ATCATGCTGACCGTCTTGCT | 29,713 |
7 | 5′-GTAGCGAAGCGGAGCGTTCTA-3′ | 28,192 | 5′-TATGGAACGAGCTGCTGGTG | 30,897 |
8 | 5′-ATTCACCAGCAGCTCGTTCC-3′ | 30,875 | 5′-TGAATGGCGTTCAGCAGGCT-3′ | 36,058 |
9 | 5′-AGCTATTCCGCCCTCACTCAA-3′ | 35,019 | 5′-TGCTAAGATTGGTCGTGTAGCT | 40,185 |
10 | 5′-TGAGGAACTTCTTGCTGACGA-3′ | 38,017 | 5′-ACTTAAAGGTGATGCTCGACGT-3′ | 43,360 |
11 | 5′-TCAGGTATTGTTCCATCCTCC-3′ | 42,662 | 5′-CAAGATACTAGTTGGAGAGCTGCT | 47,956 |
12 | 5′-CTGCAAAACCAATAGCTGTACCA-3′ | 47,220 | 5′-GGTGGTATTGCTCAACCTATTGG | 52,314 |
13 | 5′-AGAGTAGGTTGACCTGGGCCT | 50,678 | 5′-AGGTTATGGTGGGCTACAAGAT-3′ | 54,585 |
14 | 5′-TGGTCTTGTGCAGCTTGAGC-3′ | 53,477 | 5′-TATGCCCGATGATTGTTGTCCT | 58,673 |
15 | 5′-GACCAGAACGACCTCCACTA-3′ | 57,353 | 5′-TCTTGATGGTCGAGTTGATGCT-3′ | 62,669 |
16 | 5′-CACGAATACGTTGTTGCAAACCT-3′ | 60,536 | 5′-ATCGGTACTGCACTTGGTGC-3′ | 66,345 |
17 | 5′-ACCAGCCGTAAACATCTTTTCCA-3′ | 65,848 | 5′-AGTATTGGAGCAACAGGTGGA-3′ | 71,818 |
18 | 5′-AGCAGGAACAGCTTTACGAGTA-3′ | 69,175 | 5′-TTGCTAGTCTTGATGGAGATGGT-3′ | 74,902 |
19 | 5′-GTGGCACTTATTCAGTACCACCA-3′ | 74,161 | 5′-CAGAATTAGGCTTCCCATTGAACG-3′ | 79,628 |
20 | 5′-CGAAGTTTAGCAATAGGCTGCCA-3′ | 78,705 | 5′-AGGCTCTATTGGTTTGCAGGT-3′ | 83,203 |
21 | 5′-TAGCAAGACGCTCAGCTTCTC | 82,364 | 5′-GTTTGCTGAACGTCGTATGTTGAC-3′ | 87,950 |
22 | 5′-TCCATACGTTCTTCAGCTTGATTC-3′ | 86,454 | 5′-AGATGATGCTGGTGGAGAAACTT-3′ | 91,978 |
23 | 5′-GTCCAACCTTGCCAAGTAGGA-3′ | 91,910 | 5′-TGACCATCAGTACAGATGCGTCTA-3′ | 638 |
24 | 5′-AGCGTCAAGTGCTTCACTTG-3′ | 95,395 | 5′-CGAAGTCCACCATCAGCAGT-3′ | 3,167 |
