Figure 3: Photobleaching counting of V D1 bound to WT αC M and 3X WT αC M under force.
From: Force-dependent conformational switch of α-catenin controls vinculin binding
(a) Three representative time-lapse traces of the fluorescent intensity measured below individual tethered beads: 0 (WT αCM, no VD1 in solution), 1 (WT αCM, VD1 in solution) and 3 photobleaching events (3x WT αCM, VD1 in solution) were shown for each case. Dashed lines are drawn for visual guidance. (b) Histograms of the percentage of beads with different numbers of photobleaching events observed for WT αCM and 3x WT αCM constructs. The number of individual tethered beads tested for each condition is denoted by ‘n’ in figure panel. The P-value was obtained from the χ2 test with the null hypothesis that the photobleaching statistics is independent on whether force is applied.
