Figure 5: Effect of Gmip KD on the migration and distribution of new neurons in the OB.

(a) Experimental scheme. (b) Average migration speed of GFP+ cells in each layer of the OB (RMS, n=87 cells (control), n=83 cells (Gmip KD); deep GCL, n=91 cells (control), n=58 cells (Gmip KD); middle GCL, n=36 cells (control), n=15 cells (Gmip KD); superficial GCL, n=3 cells (control), n=9 cells (Gmip KD); from five mice each). **P<0.01, ****P<0.0001, t-test (see also Supplementary Movie 3). (c) Distribution of newborn neurons in the OB. Coronal brain sections of the OB at 30 d.p.e. were stained for GFP (green) and BrdU (red). The borders of the GCL, EPL and GL are marked by bold white lines. The GCL was divided into three sublayers (deep, middle (mid) and superficial (sup)), marked by thin white lines. Arrows, GFP+BrdU+ labelled cells. (d) Percentage of labelled new neurons in each layer of the OB (control, n=5 mice (1,167 cells); Gmip KD, n=5 mice (566 cells)). *P<0.05, t-test. (e) Representative examples of control or Gmip KD neurons (left panel, green; right panel, reconstructed images). Red arrowheads indicate the position of the initial branch point of the apical dendrite. (f) Distribution of initial branch points (deep GCs, n=12 cells (control), n=10 cells (Gmip KD); superficial GCs, n=56 cells (control), n=70 cells (Gmip KD); from four mice each). *P<0.05, ***P<0.001, one-way ANOVA. Data represent mean±s.e.m. (b,d). In f, data represent each point, and the mean±s.e.m. are shown as horizontal bars. Scale bars, 100 μm (c,e). EP, electroporation; i.p., intraperitoneal.