Figure 5: Correlation of isolation date with maximum likelihood root-to-tip branch length for the five major TcR lineages calculated with Path-O-Gen.

These analyses predict the origin of these clones in agreement with the BEAST analysis except for the CC23 lineage where there was a lack of temporal sampling to support tree root estimates. X axis, time in years; Y axis root-to-tip branch length in SNP per Mb. (a) CC1 lineage Tn916-1; (b) CC19 lineage Tn916-17; (c) CC17 lineage Tn5801-11; (d) CC17 lineage Tn916-12; (e) CC23 lineage Tn5801-23.