Table 4 Characteristics of clones deriving from Tn916 and Tn5801 insertions.

From: Streptococcus agalactiae clones infecting humans were selected and fixed through the extensive use of tetracycline

Clone

No. of isolates per clone

Integrative and conjugative element and site * of insertion

Recombination events within the clone †

N

CC

Sequence type

cps

   

1

1

1

V

27 (2)‡

Tn916 GI 624 kb§,||

92 kb alp; 100 kb

2

 

196, 459

IV

6

Tn916 GI 624 kb§,||

80 kb alp: 35 kb

3

 

2

IV

1

Tn5801 972 kb

—#

4

 

2

IV

1

Tn5801 972 kb

n.a.

5

 

2

II

1

Tn916 726 kb

n.a.

    

3**

 

n.a.

6

10

10

V-II

5

Tn5801 972 kb

347 kb cps operon

7

 

8

Ib

4

Tn916 846 kb

—

8

 

12

II

1

Tn916 260 kb

n.a.

9

 

10

Ib

1

Tn916 626 kb

n.a.

10

 

10

II

2

Tn916 1,255 kb

—

    

5**

 

n.a.

11

17

17

III

42 (3)‡

Tn5801 972 kb

—

12

 

17

III

23

Tn916 241 kb

—

13

 

17

III

6

Tn916 950 kb

—

14

 

17

III

2

Tn916 1,928 kb

—

15

 

17

III

1

Tn916 1,928 kb

n.a.

16

 

291

IV

5

Tn5801 52 kb

—

17

19

19

III V

20 (3)‡

Tn916 923 kb

253 kb cps operon 36 kb R5 protein

18

 

19

III

4 (1)‡

Tn916 GI 557 kb||

—

19

 

19

III

1

Tn916 2,005 kb

n.a.

20

 

28

II

2

Tn916 395 kb

—

21

 

28

II

3

Tn916 1,445 kb

—

22

 

28

II

1

Tn916 2,005 kb

n.a.

    

8**

 

n.a.

23

23

23

Ia

18 (1)‡

Tn5801 972 kb

107 kb alp; 56 kb R5 protein; 93 kb

24

 

23, 144

Ia–V

8 (1)‡

Tn916 1,928 kb

91 kb alp; 193 kb cps

25

 

23

III

1

Tn5801 972 kb

n.a.

26

 

23

Ia

1

Tn5801 972 kb

n.a.

    

8**

 

n.a.

27

26

26

V

3

Tn916 GI 1,023 kb||

—

28

 

26

V

2

Tn916 923 kb

77 kb R5 protein

29

22

22

II

2

Tn916 1787, kb

—

  1. n.a., Not applicable.
  2. *Position relative to the completely sequenced 2603 V/R genome45.
  3. †Size of the recombined regions and antigenic loci.
  4. ‡In parenthesis are indicated the number of strains predicted to have lost Tn916 or Tn5801.
  5. §Tn916 is inserted in the opposite orientation at the same site.
  6. ||Tn916 is inserted within genomic islands inserted at the reported locations relative to the 2603 V/R genome.
  7. 972 kb correspond to a hot spot of insertion of Tn5801 at the 5' end of the guaA gene.
  8. #A dash indicates no recombination event.
  9. **Number of isolates in clonal complex (CC)1, 10, 19 and 23 predicted not to have acquired Tn916 or Tn5801.
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